Yeast Cell Surface Engineering: Biological Mechanisms and Practical Applications

✍ Scribed by Mitsuyoshi Ueda

• Publisher: Springer Singapore
• Year: 2019
• Tongue: English
• Leaves: 182
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This book provides a detailed and up-to-date overview of all aspects of yeast cell surface engineering, including fundamental principles, practical strategies for the construction of engineered yeasts, as well as medical and industrial applications. The technique makes it possible to add eukaryotic modifications to the surface-displayed proteins/peptides, which is of significant value in basic and applied research. Generally referred to as an arming (molecular display) technology, it allows yeast to be used as a whole-cell biocatalyst for a range of purposes, including bio-energy production, pollutant removal, recovery of rare metal ions, and preparation of functional cells, all of which are comprehensively covered in the book. Among the medical applications discussed are in vitro antibody preparation and the production of oral vaccines. In addition, it presents the latest advances in protein engineering and high-throughput screening for directed evolution of enzymes. The book enables graduate students and researchers to gain a deeper, comprehensive understanding of the technology, and offers further inspiration for researchers and industrial experts in this rapidly evolving field.

✦ Table of Contents

Front Matter ....Pages i-viii
Front Matter ....Pages 1-1
Principle of Cell Surface Engineering of Yeast (Mitsuyoshi Ueda)....Pages 3-14
Front Matter ....Pages 15-15
Energy Production: Biomass – Starch, Cellulose, and Hemicellulose (Kouichi Kuroda)....Pages 17-28
Energy Production: Biomass – Marine (Toshiyuki Takagi)....Pages 29-41
Energy Production: Biodiesel (Chiaki Ogino, Jerome Amoah)....Pages 43-61
Cleanup of Pollution: Heavy Metal Ions and Environmental Hormones (Kouichi Kuroda)....Pages 63-72
Recovery of Rare Metal Ions (Kouichi Kuroda)....Pages 73-83
Preparation of Functional Cells: Improvement of Stress Tolerance (Kouichi Kuroda)....Pages 85-92
Bio-sensing Using Cell Surface Display: Principles and Variations of a Cell Sensor (Seiji Shibasaki)....Pages 93-106
Application of Cell Surface Engineering to Biosensing System (Shin-ichiro Suye)....Pages 107-119
Front Matter ....Pages 121-121
Engineering Antibodies and Alternative Binders for Therapeutic Uses (Wataru Aoki)....Pages 123-147
Oral Vaccine Development Using Cell Surface Display Technology (Seiji Shibasaki)....Pages 149-158
Front Matter ....Pages 159-159
Combinatorial Engineering (Mitsuyoshi Ueda)....Pages 161-173
Enzyme Evolution (Natsuko Miura)....Pages 175-185

✦ Subjects

Biomedicine; Biomedical Engineering/Biotechnology; Eukaryotic Microbiology; Antibodies; Medical Microbiology; Environmental Engineering/Biotechnology; Cell Biology