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Yeast artificial chromosome (YAC) -based genome mapping of Schistosoma mansoni

✍ Scribed by Manami Tanaka; Hirohisa Hirai; Philip T. LoVerde; Shigeo Nagafuchi; Glória R. Franco; Andrew J.G. Simpson; Sérgio D.J. Pena

Book ID
113261403
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
936 KB
Volume
69
Category
Article
ISSN
0166-6851

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Several methods have been described for using the polymerase chain reaction (PCR) to isolate fragments of DNA for genome mapping. We have developed an approach for isolating discrete fragments by amplifying DNA with single oligonucleotides (10mers) with arbitrarily selected sequences. The method is