𝔖 Bobbio Scriptorium
✦   LIBER   ✦

XRP6258-induced gene expression patterns in head and neck cancer carcinoma

✍ Scribed by George H. Yoo; Zyed Kafri; John F. Ensley; Fulvio Lonardo; Harold Kim; Adam J. Folbe; Joshua Won; Timothy Stevens; Ho-Sheng Lin

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
352 KB
Volume
120
Category
Article
ISSN
0023-852X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Objectives/Hypothesis:

XRP6258 is a novel taxoid, which has antitumor activity in preclinical mouse orthotopic and human xenograft cancer models. However, limited XRP6258 studies have been performed in head and neck squamous cell carcinoma cells (HNSCC). The objective of this study is to identify the antitumor activity of XRP6258 in HNSCC cell line models.

Methods:

HNSCC cells (HN30 and HN12) were exposed to either XRP6258 or docetaxel. XRP6258‐induced growth suppression, cell cycle arrest and apoptosis were measured. Further, XRP6258‐induced expression patterns of selected genes were compared to docetaxel‐induced expression patterns using Western blot analysis.

Results:

XRP6258 suppressed proliferation and induced G~2~M arrest and apoptosis in both of the cell lines tested. XRP6258 and docetaxel produced similar alteration in the expression of cell cycle regulators, such as cyclin A and cyclin B1. The expression of E2F and EGFR were decreased in both XRP6258 and docetaxel‐treated HNSCC cells. Finally, XRP6258 induced a greater level of bcl2 phosphorylation than docetaxel in HN12 cell line.

Conclusions:

XRP6258 appeared to have a similar mechanism of action as docetaxel in the two HNSCC cell lines studied. XRP6258 induced cell cycle arrest, growth suppression, and apoptosis by altering gene expression patterns similar to that induced by docetaxel. These preclinical experiments suggest that XRP6258 may be useful in treating HNSCC, and the aforementioned genes can potentially be used as surrogate endpoint biomarkers. Laryngoscope, 120:1114–1119, 2010

📜 SIMILAR VOLUMES

Heterogeneity of gene expression profile
Heterogeneity of gene expression profiles in head and neck cancer
✍ Jimmy Pramana; Nuno Pimentel; Ingrid Hofland; Lodewijk F. A. Wessels; Marie-Loui 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB

## Abstract ## Background. Results of gene expression profiling studies from different institutes often lack consistency. This could be due to the use of different microarray platforms and protocols, or to intratumoral heterogeneity in mRNA expression. The aim of our study was to quantify intratum

Gene expression profiling in head and ne
Gene expression profiling in head and neck squamous cell carcinoma: Clinical perspectives
✍ Benjamin Lallemant; Alexandre Evrard; Guillaume Chambon; Omar Sabra; Sophie Kach 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 108 KB 👁 2 views

## Abstract ## __Background.__ To date, more than 60 gene expression profiling (GEP) studies have been published in the field of head and neck squamous cell carcinoma (HNSCC) with variable objectives, methods, and results. ## __Methods.__ The purpose of this study was to present a state‐of‐the‐a

Expression of cancer testis antigens in
Expression of cancer testis antigens in head and neck squamous cell carcinomas
✍ David L. A. Figueiredo; Rui C. M. Mamede; Rodrigo Proto-Siqueira; Luciano Neder; 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB 👁 2 views

## Abstract ## Background. There is considerable interest in the expression of cancer testis (CT) antigens in human cancers, because they may serve as the basis for diagnostic tests or an immunologic approach to therapy, or as prognostic markers. ## Methods. On this basis, we evaluated by semiqu

Epidermal growth factor receptor gene am
Epidermal growth factor receptor gene amplification and expression in head and neck cancer cell lines
✍ Dr. Ralph R. Weichselbaum; Edward J. Dunphy; Michael A. Beckett; Allen G. Tybor; 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 485 KB 👁 1 views

We studied epidermal growth factor receptor (EGFR) gene amplification and expression in 11 early passage human head and neck carcinoma cell lines. Three cell lines demonstrated EGFR gene amplification and 10 lines showed an increase in EGFR mRNA when compared with normal keratinocytes, placenta, and

DNA methylation in the CTCF-binding site
DNA methylation in the CTCF-binding site I and the expression pattern of the H19 gene: Does positive expression predict poor prognosis in early stage head and neck carcinomas?
✍ Leda I.C.V. Esteves; Afonso C. Javaroni; Inês N. Nishimoto; José Magrin; Jeremy 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 219 KB

## Abstract Loss of allele‐specific expression by the imprinted genes __IGF2__ and __H19__ has been correlated with a differentially methylated region (DMR) upstream to the __H19__ gene. The __H19__‐DMR contains seven potential CCCTC‐binding factor (CTCF) binding sites. CTCF is a chromatin insulato