Wound Regeneration: Methods and Protocols

✍ Scribed by Hiranmoy Das

Publisher: Springer US;Humana
Year: 2021
Tongue: English
Leaves: 210
Series: Methods in Molecular Biology 2193
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Wound Regeneration: Methods and Protocols serves as a vital collection of wound model development and regeneration studies that will be of great use in both the academic and industrial fields of research.

✦ Table of Contents

Front Matter ....Pages i-xi
Investigating Epidermal Interactions Through an In Vivo Cutaneous Wound-Healing Assay (John L. Zemkewicz, Racheal G. Akwii, Constantinos M. Mikelis, Colleen L. Doçi)....Pages 1-11
A Murine Incisional Fetal Wound-Healing Model to Study Scarless and Fibrotic Skin Repair (Traci A. Wilgus)....Pages 13-21
Development of Cutaneous Wound in Diabetic Immunocompromised Mice and Use of Dental Pulp–Derived Stem Cell Product for Healing (Carl Greene, Hiranmoy Das)....Pages 23-30
Musculoskeletal Tissue Engineering Using Fibrous Biomaterials (George Tan, Yingge Zhou, Dilshan Sooriyaarachchi)....Pages 31-40
Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy (Sarah Anderson, Hiranmoy Das)....Pages 41-48
Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact (Marie-Line Fournier, Tifenn Clément, Justine Aussudre, Nikolaus Plesnila, André Obenaus, Jérôme Badaut)....Pages 49-65
Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models (Xi Li, Ning Xu Landén)....Pages 67-75
Cellular Migration Assay: An In Vitro Technique to Simulate the Wound Repair Mechanism (A K M Nawshad Hossian, George Mattheolabakis)....Pages 77-83
In Vivo Ear Sponge Lymphangiogenesis Assay (Racheal G. Akwii, Md S. Sajib, Fatema T. Zahra, Hanumantha R. Madala, Kalkunte S. Srivenugopal, Constantinos M. Mikelis)....Pages 85-96
Identification of Rho GEF and RhoA Activation by Pull-Down Assays (Md S. Sajib, Fatema T. Zahra, Racheal G. Akwii, Constantinos M. Mikelis)....Pages 97-109
Wound Matrix Stiffness Imposes on Macrophage Activation (Pu Duann, Pei-Hui Lin)....Pages 111-120
Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34+ Hematopoietic Stem Cells (Derek Barthels, Hiranmoy Das)....Pages 121-128
Electrospun Aligned Coaxial Nanofibrous Scaffold for Cardiac Repair (Divya Sridharan, Arunkumar Palaniappan, Britani N. Blackstone, Heather M. Powell, Mahmood Khan)....Pages 129-140
Generation of Myocardial Ischemic Wounds and Healing with Stem Cells (Daniela Rolph, Hiranmoy Das)....Pages 141-147
Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries (Peipei Pan, Matilda F. Chan)....Pages 149-158
Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing (Arsia Jamali, Brendan M. Kenyon, Gustavo Ortiz, Betul N. Bayraktutar, Victor G. Sendra, Pedram Hamrah)....Pages 159-174
Murine Corneal Epithelial Wound Modeling (Dhara Shah, Vinay Kumar Aakalu)....Pages 175-181
Infection-Induced Porcine Ex Vivo Corneal Wound Model to Study the Efficacy of Herpes Simplex Virus-1 Entry and Replication Inhibitors (Tejabhiram Yadavalli, Raghuram Koganti, Deepak Shukla)....Pages 183-196
Back Matter ....Pages 197-207

✦ Subjects

Life Sciences; Regenerative Medicine/Tissue Engineering; Biological Techniques; Stem Cells

📜 SIMILAR VOLUMES

Wound Regeneration and Repair: Methods a

📁 Wound Regeneration and Repair: Methods and Protocols

✍ Robert G. Gourdie (ed.), Tereance A. Myers (ed.) 📂 Library 📅 2013 🏛 Humana Press 🌐 English

In Wound Regeneration and Repair: Methods and Protocols, expert researchers in the field detail classical and cutting-edge methods for studying wound healing and regeneration. These techniques include cellular and molecular methods, genetic approaches, surgical procedures, clinical advances,

Organ Regeneration: Methods and Protocol

📁 Organ Regeneration: Methods and Protocols

✍ Benjamin M. Buehrer, Bentley Cheatham (auth.), Joydeep Basu, John W. Ludlow (eds 📂 Library 📅 2013 🏛 Humana Press 🌐 English

Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response

Axon Regeneration: Methods and Protocols

📁 Axon Regeneration: Methods and Protocols

✍ Ava J. Udvadia, James B. Antczak 📂 Library 📅 2023 🏛 Humana Press 🌐 English

This volume covers a wide range of approaches utilized to decipher cellular and molecular mechanisms that contribute to successful nerve regeneration leading to functional recovery. Chapters detail a variety of models utilizing both in vivo and in vitr

Corneal Regeneration: Methods and Protoc

📁 Corneal Regeneration: Methods and Protocols

✍ Mark Ahearne 📂 Library 📅 2020 🏛 Springer US;Humana 🌐 English

This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, h

Axon Regeneration: Methods and Protocols

📁 Axon Regeneration: Methods and Protocols

✍ Ava J. Udvadia; James B. Antczak 📂 Library 📅 2023 🏛 Springer Nature 🌐 English

This volume covers a wide range of approaches utilized to decipher cellular and molecular mechanisms that contribute to successful nerve regeneration leading to functional recovery. Chapters detail a variety of models utilizing both in vivo and in vitro approaches, physical injury models, methods fo

Axon Growth and Regeneration: Methods an

📁 Axon Growth and Regeneration: Methods and Protocols

✍ Andrew J. Murray (eds.) 📂 Library 📅 2014 🏛 Humana Press 🌐 English

Axon Growth and Regeneration: Methods and Protocols brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies. After an introductory s