Corneal Regeneration: Methods and Protocols

✍ Scribed by Mark Ahearne

Publisher: Springer US;Humana
Year: 2020
Tongue: English
Leaves: 253
Series: Methods in Molecular Biology 2145
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

✦ Table of Contents

Front Matter ....Pages i-xii
Isolation and Culture of Corneal Stromal Stem Cells (Richard M. Nagymihaly, Morten C. Moe, Goran Petrovski)....Pages 1-15
In Vitro Expansion of Corneal Endothelial Cells for Transplantation (Kim Santerre, Isabelle Xu, Mathieu Thériault, Stéphanie Proulx)....Pages 17-27
Primary Culture of Cornea-Limbal Epithelial Cells In Vitro (Finbarr O’Sullivan)....Pages 29-37
Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant (Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales et al.)....Pages 39-49
A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture (James W. Foster, Karl J. Wahlin, Shukti Chakravarti)....Pages 51-58
Gene Editing for Corneal Stromal Regeneration (Tara Moore, Connie Chao-Shern, Larry DeDionisio, Kathleen A. Christie, M. Andrew Nesbit)....Pages 59-75
Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery (Liujiang Song, Jacquelyn J. Bower, Matthew L. Hirsch)....Pages 77-102
The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea (Gaëtan Le-Bel, Pascale Desjardins, Camille Couture, Lucie Germain, Sylvain L. Guérin)....Pages 103-118
Formation of Corneal Stromal-Like Assemblies Using Human Corneal Fibroblasts and Macromolecular Crowding (Mehmet Gürdal, Gülinnaz Ercan, Dimitrios I. Zeugolis)....Pages 119-141
Preparation of Dried Amniotic Membrane for Corneal Repair (Andrew Hopkinson, Emily R. Britchford, Laura E. Sidney)....Pages 143-157
Fabrication of Corneal Extracellular Matrix-Derived Hydrogels (Mark Ahearne, Julia Fernández-Pérez)....Pages 159-168
Synthesis and Application of Collagens for Assembling a Corneal Implant (Elle Edin, Fiona Simpson, May Griffith)....Pages 169-183
Development and Validation of a 3D In Vitro Model to Study the Chemotactic Behavior of Corneal Stromal Fibroblasts (Evrim Ceren Kabak, Julia Fernández-Pérez, Mark Ahearne)....Pages 185-196
Femtosecond Laser-Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration (Neil Lagali, Mehrdad Rafat)....Pages 197-214
The Use of Animal Models to Assess Engineered Corneal Tissue (Robert Thomas Brady, Peter W. Madden)....Pages 215-230
X-Ray Diffraction Imaging of Corneal Ultrastructure (Keith M. Meek, Andrew J. Quantock, Sally Hayes, James Bell)....Pages 231-247
Back Matter ....Pages 249-250

✦ Subjects

Life Sciences; Regenerative Medicine/Tissue Engineering; Ophthalmology; Biomedical Engineering/Biotechnology

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