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Widespread Existence of Cytosine Methylation in Yeast DNA Measured by Gas Chromatography/Mass Spectrometry

✍ Scribed by Tang, Yang; Gao, Xiang-Dong; Wang, Yinsheng; Yuan, Bi-Feng; Feng, Yu-Qi

Book ID
118035943
Publisher
American Chemical Society
Year
2012
Tongue
English
Weight
328 KB
Volume
84
Category
Article
ISSN
0003-2700

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A sensitive method using gas chromatography and negative chemical ionization mass spectrometry for the detection of uracil in DNA is described. Uracil DNA glycosylase is used to specifically cleave uracil from DNA. Once removed, uracil is derivatized with 3,5-bis(trifluoromethyl)benzyl bromide and t