Wheat germ cell-free protein production system for post-genomic research

✍ Scribed by Masaki Madono; Tatsuya Sawasaki; Ryo Morishita; Yaeta Endo

• Book ID: 103851127
• Publisher: Elsevier
• Year: 2011
• Tongue: English
• Weight: 356 KB
• Volume: 28
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2010.08.009

✦ Synopsis

Genomic information becomes useful knowledge only when the structures and functions of gene products are understood. In spite of a vast array of analytical tools developed for biological studies in recent years, producing proteins at will is still a bottleneck in post-genomic studies. The cell-free protein production system we developed using wheat embryos has enabled us to produce high quality proteins for genome-wide functional and structural analyses and at the same time circumvent almost all the limitations, such as biohazards and costs, that have hampered conventional cell-free protein synthesis systems. In the present article, we introduce examples of our new wheat germ cell-free protein production system and its application to functional and structural analyses, with the focus on the former.