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Western blot analysis of the antigen in pemphigoid gestationis

โœ Scribed by SUSAN E. KELLY; B.S. BHOGAL; FENELLA WOJNAROWSKA; P. WHITEHEAD; IRENE M. LEIGH; M.M. BLACK


Book ID
108661845
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
453 KB
Volume
122
Category
Article
ISSN
0007-0963

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Sequential detection of antigens in West
โœ Nicole Theisen; Eckhard Schulze Lohoff; Kurt von Figura; Andrej Hasilik ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 794 KB

A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.