✦ LIBER ✦

Sequential detection of antigens in Western blots with differently colored products

✍ Scribed by Nicole Theisen; Eckhard Schulze Lohoff; Kurt von Figura; Andrej Hasilik

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 794 KB
Volume: 152
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90399-4

No coin nor oath required. For personal study only.

✦ Synopsis

A reliable sequential application of peroxidase and alkaline phosphatase-conjugated second antibodies to detection of two different antigens in Western blots is described. Deposits of differently colored products formed allow identification of several antigens on a single blot.

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