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Water soluble polyacrylamide as embedding medium for electron microscopy

✍ Scribed by L. Pérez-Grau; S. Muñoz-Guerra; J.A. Subirana

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
640 KB
Volume
15
Category
Article
ISSN
0739-6260

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✦ Synopsis

We have developed a method for embedding biological materials for electron microscopy in the water soluble polymer polyacrylamide (PAM). The fixed samples are infiltrated with an aqueous solution of acrylamide, which is then polymerized to form a hydrated gel. The gel is ready for sectioning after insolubilization by cross-linking with glutaraldehyde. Water is retained throughout the whole embedding process and contact of the samples with organic solvents is completely avoided. Sections are best stained with silicotungstic acid. The potential of this technique has been evaluated by detailed examination of several representative tissues. Observation ofthe native periodicity of 17 nm in PAM embedded myelin indicates a very good structural preservation. The method gives optimal results with samples which are easily infiltrated, such as pellets of cells.

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