✦ LIBER ✦

Polyethylene glycol (PEG) embedding and subsequent de-embedding as a method for the correlation of light microscopy, scanning electron microscopy, and transmission electron microscopy

✍ Scribed by Kondo, Hisatake ;Ushiki, Tatsuo

Publisher: Wiley (John Wiley & Sons)
Year: 1985
Tongue: English
Weight: 592 KB
Volume: 2
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060020507

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✦ Synopsis

The polyethylene glycol (PEG) embedding and subsequent deembedding method was applied to the observation of general tissues in scanning electron microscopy (SEMI. Resulting SEM images were of high quality. It was demonstrated that intermicroscopic correlation of images between light microscopy 0, scanning electron microscopy @EM), and transmission electron microscopy ("EM) is easily and reliably done by means of the PEG method. In particular, the exact correlation of immuno-LM with SEM is shown to be of potential value.

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