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Water permeability and the survival of frozen-thawed mouse ova and embryos

โœ Scribed by S.P. Leibo


Book ID
115849589
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
111 KB
Volume
16
Category
Article
ISSN
0011-2240

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Eight-cell mouse embryos were frozen by using glycerol, ethylene glycol, or erythritol as the cryoprotectant. The samples were slowly cooled (0.5"C/min) to temperatures between -10 and -79ยฐC before direct transfer to liquid nitrogen (-196ยฐC) and thawed slowly ( -20"C/min) or rapidly ( -500ยฐC/min). T