Survival of mouse embryos after freezing
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Miyamoto, Hajime ;Ishibashi, Takehiko
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Article
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1981
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John Wiley and Sons
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English
โ 283 KB
๐ 1 views
## Abstract Eightโcell mouse embryos were frozen by using erythritol as the cryoprotective agent. The samples were cooled slowly (1ยฐC/min) to temperatures between โ 15 and โ 75ยฐC before direct transfer into liquid nitrogen (โ 196ยฐC). The most effective concentration of erythritol for freezing of em