## Abstract Eightโcell mouse embryos were frozen by using erythritol as the cryoprotective agent. The samples were cooled slowly (1ยฐC/min) to temperatures between โ 15 and โ 75ยฐC before direct transfer into liquid nitrogen (โ 196ยฐC). The most effective concentration of erythritol for freezing of em
โฆ LIBER โฆ
Factors affecting the survival of mouse embryos during freezing and thawing
โ Scribed by H. Miyamoto
- Publisher
- Springer US
- Year
- 1986
- Tongue
- English
- Weight
- 503 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1573-7330
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The effects of cytochalasin B (CB), a microfilament inhibitor, and demecolcine (DC), a microtubule inhibitor, on freeze-thaw survival and culture survival of early cleavage stage mouse embryos, was evaluated. In the first experiment, eight-cell mouse embryos were frozen in dimethylsulfoxide (DMSO) o