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Factors influencing the outcome of embryo freezing and thawing program

โœ Scribed by Ye Ying-hui; Jin Fan; Xu Chen-ming; Xing Lan-feng

Publisher
SP Zhejiang University Press
Year
2002
Tongue
English
Weight
378 KB
Volume
3
Category
Article
ISSN
1009-3095

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## Abstract Eightโ€cell mouse embryos were frozen by using erythritol as the cryoprotective agent. The samples were cooled slowly (1ยฐC/min) to temperatures between โˆ’ 15 and โˆ’ 75ยฐC before direct transfer into liquid nitrogen (โˆ’ 196ยฐC). The most effective concentration of erythritol for freezing of em