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Volumetric and spectroscopic characterizations of the native and acid-induced denatured states of staphylococcal nuclease

✍ Scribed by Rana Filfil; Tigran V Chalikian

Book ID
115630247
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
357 KB
Volume
299
Category
Article
ISSN
0022-2836

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## Abstract Titration of a salt‐free solution of native staphylococcal nuclease by HC1 leads to an unfolding transition in the vicinity of pH 4, as determined by near‐ and far‐UV circular dichroism. At pH 2‐3, the protein is substantially unfolded. The addition of further HC1 results in a second tr