Kinetic analysis of the acid and the alkaline unfolded states of staphylococcal nuclease
β Scribed by Hueih M. Chen; J.L. You; V.S. Markin; Tian Yow Tsong
- Book ID
- 118918937
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 860 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0022-2836
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## Abstract Using highβsensitivity differential scanning calorimetry, we reexamined the thermodynamics of denaturation of staphylococcal nuclease. The denaturational changes in enthalpy and heat capacity were found to be functions of both temperature and pH. The denatured state of staphylococcal nu
Fluorescence and circular dichroism data as a function of temperature were obtained to characterize the unfolding of nuclease A and two of its less stable mutants. These spectroscopic data were obtained with a modified instrument that enables the nearly simultaneous detection of both fluorescence an