Voltammetric determination of sequence-specific DNA by electroactive intercalator on graphite electrode

✍ Scribed by Shenghui Liu; Jiannong Ye; Pingang He; Yuzhi Fang

• Book ID: 104104801
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 470 KB
• Volume: 335
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(96)00331-5

✦ Synopsis

A synthesized 24-mer single-stranded deoxyribonucleic acid (ssDNA) was covalently immobilized onto a chemicallymodified graphite electrode with surface-bound primary amino groups, using water-soluble I-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC). The immobilized ssDNA was hybridized with various concentrations of complementary DNA (cDNA) in hybridization buffer solution containing ethidium bromide (EB). The dsDNA/BB system was formed on the electrode surface. The anodic waves of the EB bound to the double-stranded DNA (dsDNA) in cyclic voltammetry were used for the determination of cDNA. The anodic peak currents (i,,) of EB were linearly related to the concentrations of cDNA between 1x10-4mgml-1 and 1x10-6mgml-'.

The detection limit was about 2x lo-'mg ml-'.