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Vitamin requirements of colpoda duodenaria

โœ Scribed by Tatum, Edward L. ;Garnjobst, Laura ;Taylor, C. V.

Publisher
Wiley (John Wiley & Sons)
Year
1942
Tongue
English
Weight
832 KB
Volume
20
Category
Article
ISSN
0095-9898

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โœฆ Synopsis

Oehler ( '21) reported growth of Colpoda cucullus on Saccharomyces exiguus in the absence of bacteria. We have been able to grow C. duodenaria satisfactorily on 6. exiguus obtained from the American Type Culture Collection.

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Previous work from this laboratory has shown that sterile culture of Colpoda duodenaria is possible in a medium containing heat-labile plasmoptyzate prepared from marine Pseudomonas (Taylor and van Wagtendonk, '41 ; van Wagtendonk and Taylor, '42) supplemented by three vitamins, nicotinic acid, thia

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## I K'rllol) I-("l'lOs Tt was showii in a prerious paper (Taylor aiid van T\'agteiidonk, '41 a ) that Colpoda duodcnarin iii pure culturc is able to grow in a sterile medium, coiiiposed of n ,i% biwvers ?cast ex%-tract aiid a plasmoptyzate of Pseudomonas fluorescens RI: 33.1. I n this medium tlic

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This organism is undoubtedly the same one used by the previous investigators and variously labelled Colpoda cucullus and C. steinii. This confusion in identification has been pointed out and clarified by Taylor and Furgason, Arch. f . Protistenk., 90, 320-339 (1938). 3The authors wish to thank Mr.