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Visualization of the Nuclei of the Basal Melanocytes of the Black Guinea-pig and of Human Skin under the Bright Field Microscope

✍ Scribed by SHUKLA, R. C.


Book ID
109644475
Publisher
Nature Publishing Group
Year
1965
Tongue
English
Weight
489 KB
Volume
207
Category
Article
ISSN
0028-0836

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Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde.