𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The fluorescence and bright field microscopic demonstration of cathepsin B in human fibroblasts

✍ Scribed by M. Graf; Ursula Leemann; F. Ruch; P. Sträuli

Publisher
Springer
Year
1979
Tongue
English
Weight
677 KB
Volume
64
Category
Article
ISSN
1432-119X

No coin nor oath required. For personal study only.

✦ Synopsis

Cathepsin B was demonstrated cytochemically in human fibroblasts with Z-Ala-Arg-Arg-2-(4-methoxy)naphtylamide as substrate. The enzyme was visualized in the bright field microscope with the diazonium salt Fast Blue B as coupling reagent and in the fluorescence microscope with 5-nitrosalicylaldehyde. With both methods cathepsin B was found in small granules distributed throughout the cytoplasm.

📜 SIMILAR VOLUMES

Fluorescent histochemical demonstration
Fluorescent histochemical demonstration of cathepsin B in the rat yolk sac
✍ P. Kugler 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 404 KB

Fluorescence demonstration of cathepsin B (E.C. 3.4.22.1) activity was performed in the yolk sac of rats near term (18th day of gestation). The enzyme demonstration was performed on freeze-dried and celloidin mounted yolk-sac sections using different substituted beta-naphthylamide derivatives as sub

Immunohistochemical demonstration of cat
Immunohistochemical demonstration of cathepsin B in the macrophages of benign and malignant lymphoid tissues
✍ J. Crocker; D. Burnett; E. L. Jones 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 696 KB

Cathepsin B has been demonstrated by immunohistochemical means in the macrophages of palatine tonsils, reactive lymph nodes and in specimens of Hodgkin's disease (HD) and non-Hodgkin's lymphomas (NHL). Two cases of genuine histiocytic lymphoma showed strong staining for the enzyme in most cells. In

The catalytically inactive precursor of
The catalytically inactive precursor of cathepsin D induces apoptosis in human fibroblasts and HeLa cells
✍ Olga Schestkowa; Dominik Geisel; Ralf Jacob; Andrej Hasilik 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 234 KB

## Abstract In several reports cathepsin D has been implicated in apoptosis. In some systems the effects of agents considered to be mediated by cathepsin D were inhibited in the presence of pepstatin A, an inhibitor of the enzyme. In other studies the effect of a mutant cathepsin D deprived of acti

Light and electron microscope demonstrat
Light and electron microscope demonstration of VIP- and enkephalin-immunoreactive nerves in the human male genitourinary tract
✍ Vaalasti, Annikki ;Tainio, Hanna ;Pelto-Huikko, Markku ;Hervonen, Antti 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 895 KB

Vasoactive intestinal polypeptide (VIP) and enkephalins were demonstrated in the nerves of the human male urogenital tract by light and electron microscope immunohistochemical techniques. Nerves containing immunoreactivity to VIP were more numerous than enkephalin-immunoreactive nerves. Both VIPand