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Visualization of Dysferlin Self-Interaction Using Fluorescence Resonance Energy Transfer and Fluctuation Correlation Spectroscopy

✍ Scribed by Han, Renzhi; Xu, Li; Pallikkuth, Sandeep; Hou, Zhanjia; Robia, Seth L.


Book ID
122223085
Publisher
Biophysical Society
Year
2011
Tongue
English
Weight
37 KB
Volume
100
Category
Article
ISSN
0006-3495

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Background: Fluorescence resonance energy transfer (FRET) is a powerful technique for measuring molecular interactions at Γ…ngstrom distances. We present a new method for FRET that utilizes the unique spectral properties of variants of the green fluorescent protein (GFP) for large-scale analysis by f