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Viscosity of chromatin solutions increases with increasing ionic strength

✍ Scribed by Rüdiger Brust

Publisher
Springer
Year
1986
Tongue
English
Weight
318 KB
Volume
11
Category
Article
ISSN
0301-4851

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✦ Synopsis

Increasing the ionic strength of rat liver chromatin solutions above 0.4 M causes increasing viscosity. This indicates transformation of the compact chromatin molecules to more elongated forms. In the range of 0.4-0.5 M ionic strength histone H1 is dissociating continuously from the chromatin and the quaternary structure chromatin unravels. At ionic strength higher than 0.5 M the viscosities of chromatin solutions are furthermore increasing due to structural deformation. Near 0.7 M ionic strength the core histones H2A and H2B begin to dissociate from the chromatin, and the opening of the nucleosome cores leads to increasing elongation of the chromatin molecules.

📜 SIMILAR VOLUMES

Increasing viscosity of E. coli chromati
Increasing viscosity of E. coli chromatin with increasing shear
✍ Douglas S. Thompson; John B. Hays; Stanley J. Gill 📂 Article 📅 1969 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 549 KB

We have observed that the viscosity of a solution of E. wli chromatin increases as the shear to which the solution is subjected is suddenly increased. This dilatant effect is opposite to the usual thixotropic non-Newtonian effects that are observed in DNA solutions. We suggest that the observed dila