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Increasing viscosity of E. coli chromatin with increasing shear

✍ Scribed by Douglas S. Thompson; John B. Hays; Stanley J. Gill


Publisher
Wiley (John Wiley & Sons)
Year
1969
Tongue
English
Weight
549 KB
Volume
7
Category
Article
ISSN
0006-3525

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✦ Synopsis


We have observed that the viscosity of a solution of E. wli chromatin increases as the shear to which the solution is subjected is suddenly increased. This dilatant effect is opposite to the usual thixotropic non-Newtonian effects that are observed in DNA solutions. We suggest that the observed dilatancy may be explained in terms of shear-induced aggregation of large DNA molecules. Conditions for aggregation are discussed in the context of the "cluster theory" of condensation.


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Increasing the ionic strength of rat liver chromatin solutions above 0.4 M causes increasing viscosity. This indicates transformation of the compact chromatin molecules to more elongated forms. In the range of 0.4-0.5 M ionic strength histone H1 is dissociating continuously from the chromatin and th