𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Viscosity and sedimentation study of sonicated DNA–proflavine complexes

✍ Scribed by Gerald Cohen; Henryk Eisenberg

Publisher
Wiley (John Wiley & Sons)
Year
1969
Tongue
English
Weight
558 KB
Volume
8
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

The inirinsic viscosity of soiiicated calf thymiis DNA (molecular weight 4-5 X lo5) increases and the sedimentation constant decreases, with increasing binding of proflavine at 0.2 ionic strength and at 25°C. The measiirements correspond to a linear increase in length of the almost rodlike DNA molecules with the amount of proflavine bonrid; independent calculations from viscosity and sedimentation measurements yield almost identical results. Over the range of r (moles of proflavine bound per moles of nricleotides) equal to zero to r = 0.13, the length increases by about 20%. This extension is compatible with the intercalation hypothesis proposed by Lerman. Density increments a t varioiis values of r, at constant chemical potential of diffusible solutes, were determined. I t was also forind that, in addition to the known isosbestic point of DNA-proflaviiie complexes at 455.5 mp, an additional ibosbestic point exists at 225.5 mp; this proved extremely ubefril for the evaluatioii of binding studies.

📜 SIMILAR VOLUMES

Fluorescence polarization study of DNA–p
Fluorescence polarization study of DNA–proflavine complexes
✍ Nerida F. Ellerton; Irvin Isenberg 📂 Article 📅 1969 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 943 KB

The binding of proflavine to L)NA has been studied by measuring the polarization and intensity of emission of DNA-dye complexes. Such measurements also permit the determination of the fluorescence of the bound dye as a function of the degree of binding. Techniques of emission spectroscopy permit the

Studies of the optical properties of the
Studies of the optical properties of the proflavine–DNA complex
✍ Hsueh Jei Li; Donald M. Crothers 📂 Article 📅 1969 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 873 KB

We report studies of the optical properties of the proflavineDNA complex, using absorbance and circular dichroism spectroscopy. From comparison of the absorption spectra of proflavine complexed with calf thymus and T2 DNA, we conclude that stacking of the dyes external to the double helix is compara

Linear dichroism characteristics of ethi
Linear dichroism characteristics of ethidium–and proflavine–supercoiled DNA complexes
✍ Charles E. Swenberg; Susan E. Carberry; Nicholas E. Geacintov 📂 Article 📅 1990 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 858 KB

A flow linear dichroism technique is utilized to study the unwinding of supercoiled DNA induced by the binding of ethidium bromide ( E B ) and proflavine ( PF) at different ratios r (drug added/DNA base). In the case of either EB or PF bound to linear calf thymus DNA, the reduced linear dichroism si

Sedimentation and viscosity of bacteriop
Sedimentation and viscosity of bacteriophage T7 DNA in presence of CH3HgOH
✍ Dieter W. Gruenwedel; Susan E. Brown 📂 Article 📅 1978 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 713 KB

The effects of increasing concentrations of methylmercuric hydroxide (CH3HgOH) on the rate of sedimentation, So, and intrinsic viscosity, [q], of T7 DNA have been studied at 20°C in 0.005,0.05, and 0.5M NapS04, respectively, whereby each salt solvent contained, in addition, 0.005M sodium borate, pH

Intrinsic viscosity of native and single
Intrinsic viscosity of native and single-stranded T7 DNA and its relationship to sedimentation coefficient
✍ Alan H. Rosenberg; F. William Studier 📂 Article 📅 1969 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 501 KB

The intrinsic viscosity and sedimentation coefficient of native and single-stranded T7 DNA have been determined a t 25°C as a function of ionic strength in neutral and alkaline NaCl. The relationship between [q] and S;,,, is well represented by the Mandelkern-Flory equation over the entire range of