Intrinsic viscosity of native and single-stranded T7 DNA and its relationship to sedimentation coefficient
✍ Scribed by Alan H. Rosenberg; F. William Studier
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1969
- Tongue
- English
- Weight
- 501 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
The intrinsic viscosity and sedimentation coefficient of native and single-stranded T7 DNA have been determined a t 25°C as a function of ionic strength in neutral and alkaline NaCl. The relationship between [q] and S;,,, is well represented by the Mandelkern-Flory equation over the entire range of conditions between 0.0013 and 1M Na+. An apparent discrepancy between the two methods at moderate to high ionic strengths is probably due to a change in V with ionic strength. It appears that [q] is a more sensitive and reliable measure of molecular expansion for native DNA, but S",,, is a better index of conformational change in single strands, since [q] becomes too small to measure conveniently at high ionic strengths. At moderate to high ionic strengths, denaturation leads to a decrease in [q], although unfolded single strands retain considerable viscosity. At sufficiently low ionic strength, the intrinsic viscosity of the single strands becomes higher than that of native DNA, and the effective volume of a single strand approaches that of the native molecule.