The effects of increasing concentrations of methylmercuric hydroxide (CH3HgOH) on the rate of sedimentation, So, and intrinsic viscosity, [q], of T7 DNA have been studied at 20ยฐC in 0.005,0.05, and 0.5M NapS04, respectively, whereby each salt solvent contained, in addition, 0.005M sodium borate, pH
Viscosity and sedimentation of the DNA from bacteriophages T2 and T7 and the relation to molecular weight
โ Scribed by Crothers, D.M.; Zimm, B.H.
- Book ID
- 122825932
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 748 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0022-2836
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๐ SIMILAR VOLUMES
Polyelectrolyte expansion effects on high molecular weight bacteriophage DNA have bcen studied by examining the influence of simple salt concentration upon the intrinsic viscosity, [TI. The viscosity-molecular weight exponent a in the expression [q] = KM. diminishes from 0.8 in 0.005M simple salt to
The intrinsic viscosity and sedimentation coefficient of native and single-stranded T7 DNA have been determined a t 25ยฐC as a function of ionic strength in neutral and alkaline NaCl. The relationship between [q] and S;,,, is well represented by the Mandelkern-Flory equation over the entire range of