Virus-Induced Gene Silencing in Plants: Methods and Protocols

✍ Scribed by Vincent Courdavault, Sébastien Besseau

• Publisher: Springer US;Humana
• Year: 2020
• Tongue: English
• Leaves: 267
• Series: Methods in Molecular Biology 2172
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xii
Virus-Induced Gene Silencing in Lilies Using Cucumber Mosaic Virus Vectors (Keisuke Tasaki, Masumi Yamagishi, Chikara Masuta)....Pages 1-13
Virus-Induced Gene Silencing in Poaceae Using a Foxtail Mosaic Virus Vector (Ying-Wen Huang, Chao-Yuan Chang, Yau-Heiu Hsu)....Pages 15-25
Virus-Induced Gene Silencing (VIGS) in Chili Pepper (Capsicum spp.) (Magda Lisette Arce-Rodríguez, Neftalí Ochoa-Alejo)....Pages 27-38
Virus-Induced Gene Silencing in Diploid and Tetraploid Potato Species (Jinping Zhao, Haolang Jiang, Guanyu Wang, Zonghua Wang, Jingao Dong, Junqi Song)....Pages 39-50
Virus-Induced Gene Silencing (VIGS) in Cassava Using Geminivirus Agroclones (Syed Shan-e-Ali Zaidi, Kumar Vasudevan, Ezequiel Matias Lentz, Hervé Vanderschuren)....Pages 51-64
Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum) (Maxime Chantreau, Godfrey Neutelings)....Pages 65-74
Virus-Induced Gene Silencing to Investigate Alkaloid Biosynthesis in Opium Poppy (Rongji Chen, Xue Chen, Jillian M. Hagel, Peter J. Facchini)....Pages 75-92
A Biolistic-Mediated Virus-Induced Gene Silencing in Apocynaceae to Map Biosynthetic Pathways of Alkaloids (Pamela Lemos Cruz, María Isabel Restrepo, Thomas Dugé de Bernonville, Audrey Oudin, Thibaut Munsch, Arnaud Lanoue et al.)....Pages 93-110
Virus-Induced Gene Silencing in Nepeta (Lira Palmer, Sarah E. O’Connor)....Pages 111-121
Virus-Induced Gene Silencing in Sweet Basil (Ocimum basilicum) (Rajesh Chandra Misra, Shubha Sharma, Anchal Garg, Sumit Ghosh)....Pages 123-138
Virus-Induced Gene Silencing for Functional Genomics in Withania somnifera, an Important Indian Medicinal Plant (Dikki Pedenla Bomzan, H. B. Shilpashree, P. Anjali, Sarma Rajeev Kumar, Dinesh A. Nagegowda)....Pages 139-154
A Prunus necrotic ringspot virus (PNRSV)-Based Viral Vector for Characterization of Gene Functions in Prunus Fruit Trees (Hongguang Cui, Yinzi Li, Aiming Wang)....Pages 155-163
Virus-Induced Gene Silencing in Olive Tree (Oleaceae) (Konstantinos Koudounas, Margarita Thomopoulou, Elisavet Angeli, Dikran Tsitsekian, Stamatis Rigas, Polydefkis Hatzopoulos)....Pages 165-182
ALSV-Based Virus-Induced Gene Silencing in Apple Tree (Malus × domestica L.) (Carolina Werner Ribeiro, Thomas Dugé de Bernonville, Gaëlle Glévarec, Arnaud Lanoue, Audrey Oudin, Olivier Pichon et al.)....Pages 183-197
Virus-Induced Gene Silencing for Functional Analysis of Flower Traits in Petunia (Shaun R. Broderick, Laura J. Chapin, Michelle L. Jones)....Pages 199-222
Virus-Induced Gene Silencing in Rose Flowers (Huijun Yan, Zhao Zhang, Jean-Louis Magnard, Benoît Boachon, Sylvie Baudino, Kaixue Tang)....Pages 223-232
Virus-Induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seed Coat: Description of an Effective Procedure Using the transparent testa 2 Gene as a Selectable Marker (Christophe Hano, Samantha Drouet, Eric Lainé)....Pages 233-242
Virus-Based microRNA Silencing in Plants (Jinping Zhao, Guanyu Wang, Haolang Jiang, Tingli Liu, Jingao Dong, Zonghua Wang et al.)....Pages 243-257
Back Matter ....Pages 259-261

✦ Subjects

Life Sciences; Plant Sciences; Plant Genetics and Genomics