Plant Gene Silencing: Methods and Protocols
β Scribed by Kirankumar S. Mysore, Muthappa Senthil-Kumar (eds.)
- Publisher
- Humana Press
- Year
- 2015
- Tongue
- English
- Leaves
- 300
- Series
- Methods in Molecular Biology 1287
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
β¦ Table of Contents
Front Matter....Pages i-xi
Front Matter....Pages 1-1
Front Matter....Pages 3-23
Front Matter....Pages 25-41
Back Matter....Pages 43-60
....Pages 61-79
β¦ Subjects
Plant Genetics & Genomics
π SIMILAR VOLUMES
<p><p>This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing,
<p><p>This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing expe
<p><p>This volume presents protocols that analyze and explore gene regulatory networks (GRNs) at different levels in plants. This book is divided into two parts: Part I introduces different experimental techniques used to study genes and their regulatory interactions in plants. Part II highlights di
<p><p>Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plantβs development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of