Virulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane
✍ Scribed by Mirja Wessel; Sonja Klüsener; Julia Gödeke; Christiane Fritz; Stephanie Hacker; Franz Narberhaus
- Book ID
- 110833202
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 520 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0950-382X
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Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant
The effect of a large number of Tn3 insertions in the vir region of the Ti plasmid pTiA6NC on the virulence of Agrobacterium was determined. The Vir- insertions were mapped in three of the five loci that have been defined previously. Merodiploid Rec- strains carrying one insertion mutation on the Ti