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Viral integration and fragile sites in human papillomavirus-lmmortalized human keratinocyte cell lines

โœ Scribed by Patricia P. Smith; Cynthia L. Friedman; Eileen M. Bryant; James K. McDougall

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
797 KB
Volume
5
Category
Article
ISSN
1045-2257

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โœฆ Synopsis

Human papillomavirus (HPV) types I 6 and 18 integration sites were mapped in six HPV-immortalized human keratinocyte cell lines by fluorescence in situ hybridization (FISH). Mapping of HPV sequences in these cell lines revealed that HPV integration varied in copy number and location but that integration sites were stable over extended passages in culture. Integration occurred at different sites throughout the genome and did not correspond to the location of specific cellular genes. However, integration sites were consistent with integration near or within known fragile sites in five of the six cell lines. Induction of aphidicolin-sensitive fragile sites in one cell line prior to in situ hybridization revealed that integrated HPV DNA was disrupted by fragile-site expression, suggesting that integration occurred within a fragile site. Genes Chrom Cancer 5: 150-157 ( 1 992).

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