Distinct P-cadherin expression in cultured normal human keratinocytes and squamous cell carcinoma cell lines

✍ Scribed by Wakita, Hisashi; Shirahama, Shigeho; Furukawa, Fukumi

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 358 KB
• Volume: 43
• Category: Article
• ISSN: 1059-910X
• DOI: 10.1002/(sici)1097-0029(19981101)43:3<218::aid-jemt3>3.0.co;2-s

✦ Synopsis

Spatially regulated expression of E (epithelial)-and P (placental)-cadherins is crucial for maintaining normal epidermal architecture. In cutaneous squamous cell carcinomas (SCCs), aberrant P-cadherin expression is often observed in ''squamoid'' cancer cells, whereas E-cadherin expression in cancer cells is generally reduced. Therefore, it is plausible that SCC cells have acquired the ability to express P-cadherin and that P-cadherin plays a role in tumor progression. To address the issue, the in vitro effect of extracellular calcium on differentiation is a good model for investigating P-cadherin in normal and neoplastic skin. With elevations in extracellular calcium, human SCC cell line (DJM-1) cells initiate de novo synthesis of P-cadherin and express P-cadherin on the cell surface, whereas in normal human keratinocytes, P-cadherin expression on the cell surface is enhanced via the translocation from the cytosol to the cell membrane and/or the stabilization of P-cadherin at the cell surface. DJM-1 cells maintain P-cadherin expression on the cell surface at high levels for over 4 days after calcium elevation, whereas normal human keratinocytes cannot sustain cell surface P-cadherin when the cells are cultured in high calcium for more than 2 days. P-cadherin synthesis in DJM-1 cells is regulated at translational levels by extracellular calcium concentrations. SCC cells have the ability to produce P-cadherin by a mechanism not observed in normal keratinocytes, which might relate to the aberrant expression of P-cadherin in SCC of the skin.