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Viability of human diploid cells as a function of in vitro age

✍ Scribed by George S. Merz Jr.; John D. Ross

Publisher
John Wiley and Sons
Year
1969
Tongue
English
Weight
190 KB
Volume
74
Category
Article
ISSN
0021-9541

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✦ Synopsis

The fraction of cells capable of division was determined for 1) population of the human diploid cell strains, W138 after different numbers of subcultivations in uitro and 2 ) a single population of W138 cells at intervals throughout its entire in vitro lifespan. In both cases the percentage of cells capable of division decreased with increasing age in tissue culture. The rate and the magnitude of the decrease is sufficient to account for the limited in uitro lifespan reported by other investigators.

Furthermore, the decrease in the fraction of cells capable of division in similar in some respects of senescence among human populations.

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