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Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability

✍ Scribed by J. M. Soria; C. Martínez Ramos; O. Bahamonde; D. M. García Cruz; M. Salmerón Sánchez; M. A. García Esparza; C. Casas; M. Guzmán; X. Navarro; J. L. Gómez Ribelles; J. M. García Verdugo; M. Monleón Pradas; J. A. Barcia

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
349 KB
Volume
83A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

A series of polymeric biomaterials including poly (methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007

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