Viability assessment of dog spermatozoa using flow cytometry
✍ Scribed by A.I Peña; L.A Quintela; P.G Herradón
- Book ID
- 117271574
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 513 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0093-691X
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The ability of a flow cytometer to rapidly assess microbial viability was investigated using three vital stains: rhodamine 123 (Rh123); 3,3'-dihexyloxacarbocyanine iodide [DiOC6(3)] and fluorescein diacetate (FDA). Rh123 was found to clearly differentiate viable from non-viable bacteria. The methodo
Methods are given for the preparation and staining of human spermatozoa for flow cytometric DNA measurements. Using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes an effective decondensation of the sperm chromatin and a DNA-proportional uptake of fluorochr