Variation in high molecular weight glutenin subunits ofTriticum aestivumandT. turgidumssp.dicoccoides
โ Scribed by P. Kolster; C. F. Krechting; W. M. J. van Gelder
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 398 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0014-2336
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โฆ Synopsis
Two-dimensional electrophoresis revealed biochemical variation for glu-B1 high molecular weight (HMW) glutenin subunit 7 present in Dutch wheat (Triticum aestivum) varieties . This variation was not recognized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) . In T turgidum ssp . dicoccoides, HMW glutenin subunits were detected which do not occur in varieties . Some of these subunits show similar relative mobilities during SDS-PAGE as subunits present in varieties and as such unambiguous identification is not possible . Before species related to T aestivum are used in a breeding programme, their HMW glutenin subunits should be identified by two-dimensional electrophoresis, in order to avoid the introduction of HMW glutenin subunits, of which the influence on bread-making quality is not known .
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The diversity of high molecular weight (HMW) glutenin subunits of 502 varieties of durum wheat (Triticum durum) from 23 countries was studied using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-nine types of patterns were observed with 18 mobility bands. A total of 18
Restriction enzyme digests of DNA from nullisomic-tetrasomic and intervarietal chromosome substitution lines of wheat were probed with a high molecular weight (HMW) glutenin cDNA. Three restriction endonucleases were used to investigate restriction-fragment differences among five wheat varieties. Th