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Validation of suitable endogenous control genes for quantitative PCR analysis of microRNA gene expression in a rat model of endometrial cancer

✍ Scribed by Sanja Jurcevic, Björn Olsson, Karin Klinga-Levan


Book ID
120685487
Publisher
BioMed Central
Year
2013
Tongue
English
Weight
194 KB
Volume
13
Category
Article
ISSN
1475-2867

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Identification of suitable endogenous co
✍ Li, Dongwei; Liu, Hongyu; Li, Yunsheng; Yang, Manman; Qu, Changqing; Zhang, Yunh 📂 Article 📅 2014 🏛 Springer 🌐 English ⚖ 321 KB

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become the preferred technique for studying low-abundance RNA expression. Proper normalization is a critical but often underappreciated aspect of quantitative gene expression analysis; popular endogenous control genes are usu