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Identification of suitable endogenous control genes for quantitative RT-PCR analysis of miRNA in bovine solid tissues

✍ Scribed by Li, Dongwei; Liu, Hongyu; Li, Yunsheng; Yang, Manman; Qu, Changqing; Zhang, Yunhai; Liu, Ya; Zhang, Xiaorong


Book ID
125498625
Publisher
Springer
Year
2014
Tongue
English
Weight
321 KB
Volume
41
Category
Article
ISSN
0301-4851

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Identification of suitable endogenous co
✍ Li, Dongwei; Liu, Hongyu; Li, Yunsheng; Yang, Manman; Qu, Changqing; Zhang, Yunh πŸ“‚ Article πŸ“… 2014 πŸ› Springer 🌐 English βš– 321 KB

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become the preferred technique for studying low-abundance RNA expression. Proper normalization is a critical but often underappreciated aspect of quantitative gene expression analysis; popular endogenous control genes are usu