UVC-INDUCED CELL DEATH OF IL-2-DEPENDENT HUMAN LYMPHOCYTES

✍ Scribed by E Radziszewska; K Piwocka; J Skierski; E Sikora

• Book ID: 102567549
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 310 KB
• Volume: 23
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1998.0341

✦ Synopsis

We compared the in vitro propensity of human IL-2-dependent lymphocytes (young proliferating and senescent non-proliferating), and resting peripheral blood lymphocytes (PBLs) to undergo UVC-induced apoptosis. The activities of AP-1 (activator protein-1), CRE (cAMP response element) and OCT-1 (octamer-1) transcription factors in all lymphocytes were also assessed. At 24 h after UVC treatment, half of young proliferating T lymphocytes and about a quarter of PBLs and senescent non-proliferating cells were apoptotic, as shown by flow cytometry. However, only in young lymphocytes were both typical DNA 'ladder' and Bcl-2 downregulation evident. The AP-1 transcription factor was activated by UVC in IL-2-dependent young and senescent, but not resting lymphocytes. Taken together, the data show different propensities of resting, proliferating and senescent human lymphocytes to undergo UVCinduced apoptosis and AP-1 activation.