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Utilization of high-accuracy FTICR-MS data in protein quantitation experiments

✍ Scribed by Martin Strohalm; Petr Novak; Petr Pompach; Petr Man; Daniel Kavan; Matthias Witt; Petr Dzubak; Marian Hajduch; Vladimir Havlicek

Book ID
102378257
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
213 KB
Volume
44
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Human acute T‐lymphoblastic leukemia cell line (CEM) treated with cisplatin, and the stable isotope labeling by amino acids in cell culture (SILAC) strategy were used to present an improved method of data processing in high‐accuracy mass spectrometry (MS). By using peptide mass fingerprinting with low mass tolerance, we were able to utilize far more data retained in MS scans which would normally be missed by a standard processing method. This new way of data interpretation results in an improvement of the relevance of quantitation experiments and enabled us to search and quantify different types of posttranslational modifications. Furthermore, we used this technique to distinguish among different protein isoforms, commonly returned by Mascot search engine. Copyright © 2009 John Wiley & Sons, Ltd.

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