✍ Scribed by Wagner, Betty Jean ;Miović, Margaret L. ;Gibson, Jane
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Chromatium was unable to use any amino acids as carbon source, and only glutamate, glutamine and aspartate as nitrogen sources.
Addition of individual amino acids to otherwise adequate medium at concentrations of 5 mM did not inhibit growth.
Radioactive leucine and valine were incorporated only into cell protein during growth. Only leucine was labelled following growth in radioactive leucine but both valine and leucine were labelled following growth in valine.
After labelled alanine incorporation, radioactivity was found in the lipid and nucleic acid fractions as well as in protein, but over 700/0 of the counts recovered in the protein fraction were in alanine.
Growth and short-term uptake experiments showed reduction of leucine uptake in the presence of valine or isoleucine. Uptake of leueine and alanine was lightstimulated and reduced by chloramphenicol.
No evidence of induction of amino acid uptake systems was found, and amino acid converting enzymes, as well as some key biosynthetic activities, were hOt,affected by growth in amino-acid-containing media. 7. It is suggested that the limited use made of amino acids by Chromatium is due
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Utilization of dissolved free and combined amino acids (DFAA and DCAA) by two types of bacterial isolates in artificial seawater was investigated. One type of isolate had proteolytic activity and the other did not. Both types of isolates utilized DFAA in artificial seawater, but there was some selec