Using the Yeast Interaction Trap and Other Two-Hybrid-Based Approaches to Study Protein-Protein Interactions
✍ Scribed by Garabet G. Toby; Erica A. Golemis
- Book ID
- 115639291
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 240 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1046-2023
No coin nor oath required. For personal study only.
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The yeast two-hybrid technique provides a general approach for cloning cDNAs merely by exploiting the ability of their encoded proteins to bind to a protein of interest. The technique therefore offered a useful access to the analysis of the mechanisms of cell death at the initial stage of their stud
## Abstract The analysis of protein‐protein interactions is a key focus of proteomics efforts. The yeast two‐hybrid system (Y2H) has been the most commonly used method in genome‐wide searches for protein interaction partners. However, the throughput of the current yeast two‐hybrid array approach is