𝔖 Bobbio Scriptorium
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Using the method of depth reconstruction from focusing for microscope images

✍ Scribed by Averkin, A. N.; Potapov, A. S.

Book ID
115402860
Publisher
Optical Society of America
Year
2011
Tongue
English
Weight
523 KB
Volume
78
Category
Article
ISSN
1070-9762

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A confocal laser scanning microscope (CLSM) enables us to capture images from a biological specimen in different depths and obtain a series of precisely registered fluorescent images. However, images captured from deep layers of the specimen may be darker than images from the topmost layers because