Using neutron protein crystallography to understand enzyme mechanisms

✍ Scribed by Glusker, Jenny P. ;Carrell, H. L. ;Kovalevsky, Andrey Y. ;Hanson, Leif ;Fisher, S. Zoe ;Mustyakimov, Marat ;Mason, Sax ;Forsyth, Trevor ;Langan, Paul

• Book ID: 104478578
• Publisher: International Union of Crystallography
• Year: 2010
• Tongue: English
• Weight: 593 KB
• Volume: 66
• Category: Article
• ISSN: 0907-4449
• DOI: 10.1107/s0907444910027915

✦ Synopsis

A description is given of the results of neutron diffraction studies of the structures of four different metal-ion complexes of deuterated D-xylose isomerase. These represent four stages in the progression of the biochemical catalytic action of this enzyme. Analyses of the structural changes observed between the various three-dimensional structures lead to some insight into the mechanism of action of this enzyme.