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Using an Inverse PCR Method to Clone the Wheat Cytokinin Oxidase/Dehydrogenase GeneTaCKX1

โœ Scribed by De-Shun Feng; Hong-Gang Wang; Xian-Sheng Zhang; Ling-Rang Kong; Ji-Chun Tian; Xing-Feng Li

Publisher
Springer
Year
2008
Tongue
English
Weight
661 KB
Volume
26
Category
Article
ISSN
0735-9640

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โœ Mirjam P. Does; Ben M. M. Dekker; Marcel J. A. Groot; Remko Offringa ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 397 KB

Agrobacterium-mediated transformation of plants is known to result in transgenic plants with a variable number of integrated T-DNA copies . Our aim was to obtain transgenic tobacco plants containing one integrated T-DNA copy per genome. Therefore, a quick method was developed to estimate the T-DNA c