✦ LIBER ✦
A quick method to estimate the T-DNA copy number in transgenic plants at an early stage after transformation, using inverse PCR
✍ Scribed by Mirjam P. Does; Ben M. M. Dekker; Marcel J. A. Groot; Remko Offringa
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 397 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0167-4412
No coin nor oath required. For personal study only.
✦ Synopsis
Agrobacterium-mediated transformation of plants is known to result in transgenic plants with a variable number of integrated T-DNA copies . Our aim was to obtain transgenic tobacco plants containing one integrated T-DNA copy per genome. Therefore, a quick method was developed to estimate the T-DNA copy number of young transgenic plantlets within 10 weeks after transformation. Inverse polymerase chain reaction (IPCR) was used to amplify junction fragments, i.e. plant genomic DNA sequences flanking the known T-DNA sequences .