Use of the polymerase chain reaction for Salmonella detection
โ Scribed by J. Kwang; E.T. Littledike; J.E. Keen
- Book ID
- 111190177
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 381 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0266-8254
No coin nor oath required. For personal study only.
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Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular bea