Use of the Ninhydrin Reaction for Quantitative Estimation of Amino Groups in Insoluble Specimens
โ Scribed by KENDALL, P. A.
- Book ID
- 109625934
- Publisher
- Nature Publishing Group
- Year
- 1963
- Tongue
- English
- Weight
- 267 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0028-0836
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๐ SIMILAR VOLUMES
A method for the determination of free amino groups on solid supports is proposed. The method is based on the reaction of the matrix with excess 2,4,6-trinitrobenzenesulfonic acid (TNBS) and subsequent quantitative determination of unreacted TNBS by reaction with glycine. The method is simple and re
A sensitive modification of the ninhydrin-CO 2 method involving the gas chromatographic determination of the total protein and amino acid content of sediment is described . The method gives a linear response over the amino acid concentration range 10 -5 M to 4 X 10-2 M . It can be used for whole sed