We have established procedures for reisolating a transfected gene from mammalian cells by selection in Escherichia coli for the function of the gene product using the Herpes simplex virus thymidine kinase gene as a model. Rescue of the gene is accomplished by three different methods. The tk gene is
Use of the Escherichia coli chromosomal DHFR gene as selection marker in mammalian cells
β Scribed by Fred A.M. Asselbergs; Roland Widmer
- Book ID
- 119132261
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 518 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0168-1656
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Recent demonstrations of loss of heterozygosity in a wide variety o f human cancers suggest that large multilocus genetic deletions (presumably including tumor suppressor genes) constitute a major class of genetic alteration in human carcinogenesis. Here we show that a bifunctional fusion gene (Hytk
Assimilation of iron via aerobactin by Escherichia coli requires functions encoded by certain ColV plasmids and by the chromosomal fhu region. Mutants obtained by insertion of the transposons Mu d1 (Ap lac) and Tn5 into ColV-K311 revealed that the aerobactin genes are transcribed from the locus that