๐”– Bobbio Scriptorium
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Use of tetrameric antibody complexes to stain cells for flow cytometry

โœ Scribed by Albertus W. Wognum; Terry E. Thomas; Peter M. Lansdorp

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
665 KB
Volume
8
Category
Article
ISSN
0196-4763

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A simple system is described for producing quasistable complexes between biotinylated antibodies and fluorochrome-labeled streptavidin. Optimal complexes were produced by mixing biotinylated antibody and phycoerythrin-streptavidin together at a molar ratio of 1:1 for 2 min, followed by the addition